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Infrastructure

The Rossjohn lab currently has the following infrastructure.

The Protein Crystallography Unit was established at Monash University in 2002.  The Unit was generously funded by the following organisations, and their support was very much appreciated:
The Ian Potter Foundation
The National Health & Medical Research Council
The Victorian State Government
Monash University

The Unit comprises a Crystallization and X-ray diffraction laboratory, and each research group that uses the core infrastructure has their own laboratory for the expression and purification of proteins.  All crystallographic-related computing is processed via a Mac-cluster.

1) The Crystallization Laboratory
The crystallization room is temperature-controlled, with the crystallization trays being stored on anti-vibration shelving.  The laboratory houses three Leica stereomicroscopes and a Crystallization robot.

2) The X-ray diffraction laboratory
The X-ray diffraction laboratory abuts the crystallization laboratory. All in-house X-ray measurements are made using on of two Rikagu RU-3HBR rotating anode generator with helium purged OSMIC focussing mirrors as an X-ray source. One of the generators is also equiped for the use of a chromium anode.  Data are collected using an R-AXIS IV++ detector. Crystals are routinely flash frozen (to a temperature of 100K) prior to data collection using the inverse phi geometry with an Oxford cryosystem
In addition, the laboratory frequently makes visits to synchrotron facilities in the United States and Australia, where more intense X-rays are available.
Advanced Photon Source { http://www.aps.anl.gov}

Australian synchrotron {http://www.synchrotron.vic.gov.au }

For biophysical measurements of protein-protein interactions, the laboratory also possesses a Biacore 3000, and also has routine access to an isothermal titration calorimeter.

Within the Rossjohn lab., proteins are regularly expressed in prokaryotic and eukaryotic expression systems, and accordingly possess the infrastructure to achieve this.  We also possess a wide range of FPLCs to purify the proteins. The Protein Crystallography Unit also has access to the Protein Production Unit {http://bioplatforms.monash.edu.au/bioplatforms/ppu.html}.

 

In 2009 there was a significant upgrade to the X-ray and Crystallization infrastructure.  These upgrades are funded by Monash University, State and Federal Governments, Australian Regenerative Medicine Institute (ARMI) and the Australian Research Council.

The CrystalMation Robotics are part of the infrastructure of the Protein Crystallography Unit.

The robotics are manged by Dr Danuta Maksel assited by Dr Robyn Gray.

The x-ray diffraction laboratory in B76 which houses the two Rigaku generators